Journal: iScience

Article Title: Temporal and spatial dynamics of immune cells in spontaneous liver transplant tolerance

doi: 10.1016/j.isci.2023.107691

Figure Lengend Snippet: List of metal-labeled antibodies used for mass cytometry

Article Snippet: Anti-CD11c , Fluidigm , 3142003C.

Techniques:

Journal: iScience

Article Title: Temporal and spatial dynamics of immune cells in spontaneous liver transplant tolerance

doi: 10.1016/j.isci.2023.107691

Figure Lengend Snippet: Immunohistochemical staining of different cell markers in hepatic grafts Different immune cell markers (B220, CD3, CD8, Ly6G, I-A k , CD11c, and F4/80) were stained in hepatic grafts in Naïve control (Naïve), syngeneic control (Syn), postoperative days (POD) 7, POD14, POD30, and POD100 groups. Image data are representative of three independent experiments. Scale bar = 200 μm.

Article Snippet: Anti-CD11c , Fluidigm , 3142003C.

Techniques: Immunohistochemistry, Staining

Journal: iScience

Article Title: Temporal and spatial dynamics of immune cells in spontaneous liver transplant tolerance

doi: 10.1016/j.isci.2023.107691

Figure Lengend Snippet:

Article Snippet: Anti-CD11c , Fluidigm , 3142003C.

Techniques: Transplantation Assay, Recombinant, Staining, Cell Isolation, Software, Cytometry, Flow Cytometry

Journal: The Journal of investigative dermatology

Article Title: Overexpression of cathepsin S induces chronic atopic dermatitis in mice.

doi: 10.1038/jid.2011.404

Figure Lengend Snippet: Figure 4. Proteinase-activated receptor-2 (PAR-2) expression and inflammatory cell infiltration in dorsal skin. Real-time PCR analysis of (a) cathepsin S (CTSS) and (b) PAR-2 expression. Diamonds, C57BL/6 WT mice; squares and triangles, CTSS-overexpressing TG mice (n ¼ 4). The average value for WT mice was set to 1. *Po0.05, diamonds and squares; #Po0.05, ##Po0.01, squares and triangles. (c) Western blot analysis of dorsal skin tissue homogenates with antibodies specific for CTSS, PAR-2, and b-actin (arrow). Immunostaining of dorsal skin with antibodies specific for (d) CTSS, PAR-2, and CD11c, as well as (e) major histocompatibility complex (MHC) class II, CD4 (CD4 þ T-cell marker), and CD68 (macrophage marker). Markers of mature dendritic cells: CD11c and MHC class II. Bar ¼ 50 mm. Positive cells show brown staining. TG, CTSS-overexpressing transgenic mice; WT, C57BL/6 wild-type mice.

Article Snippet: The following antibodies were used: anti-mouse cytokeratin 10 (K10) monoclonal antibody (Covance, Princeton, NJ), anti-rabbit Ki67 monoclonal antibody (Thermo, Hudson, CA), anti-mouse proliferat- ing cell nuclear antigen monoclonal antibody, anti-goat CTSS poly- clonal antibody, anti-rabbit PAR-2 polyclonal antibody, anti-mouse MHC class II monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA), anti-hamster CD11c monoclonal antibody, anti-rabbit CD4 polyclonal antibody (Novus Biologicals, Littleton, CO), and anti-rat macrophage monoclonal antibody (Abcam, Cambridge, UK).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Immunostaining, Immunopeptidomics, Marker, Staining, Transgenic Assay

Journal: Immunity

Article Title: Pro-inflammatory Aorta-Associated Macrophages Are Involved in Embryonic Development of Hematopoietic Stem Cells

doi: 10.1016/j.immuni.2019.05.003

Figure Lengend Snippet:

Article Snippet: Anti-Mouse CD11c (clone N418)-162Dy , Fluidigm , Cat# 3162017C.

Techniques: Purification, Recombinant, Software, Control, High Throughput Screening Assay, Sequencing

Journal: Journal of Diabetes Research

Article Title: Attenuation of Diabetic Nephropathy in Diabetic Mice by Fasudil through Regulation of Macrophage Polarization

doi: 10.1155/2020/4126913

Figure Lengend Snippet: Fasudil inhibited M1 macrophage polarization and proinflammatory cytokines in renal tissues of diabetic mice. (a) Representative immunohistochemical staining of CD11c (a marker for M1 macrophages; red arrows), IL-6, and TNF- α in renal tissues. (b) Quantitative analysis for the number of M1 macrophages in renal tissue specimens. Data are expressed as mean ± SD, ∗ P < 0.05 vs. the Ctrl group; # P < 0.05 vs. the DM group. (c) Quantitative analysis of the protein expression of IL-6 in renal tissue specimens. Data are expressed as mean ± SD, ∗ P < 0.05 vs. the Ctrl group; # P < 0.05 vs. DM group. (d) Quantitative analysis of the protein expression of TNF- α in renal tissue specimens. Data are expressed as mean ± SD, ∗ P < 0.05 vs. the Ctrl group; # P < 0.05 vs. the DM group.

Article Snippet: CD68, CD11c, and CD206 antibodies were all purchased from the ProteinTech Group (USA).

Techniques: Immunohistochemical staining, Staining, Marker, Expressing

Journal: Mediators of Inflammation

Article Title: Aspirin Disrupts the Crosstalk of Angiogenic and Inflammatory Cytokines between 4T1 Breast Cancer Cells and Macrophages

doi: 10.1155/2018/6380643

Figure Lengend Snippet: Effect of aspirin on M1 and M2 macrophage subtypes following LPS stimulation and coculture with 4T1 cells. Macrophages were incubated in the presence or absence of aspirin for 72 h and cultured in either fresh medium as a control, the presence of LPS for the last 24 h of the incubation, or cocultured with 4T1 cells for 72 h. (a) Histogram plots, (b) fluorescent intensity plots, and (c) quantitative data were presented. The immunofluorescent intensity of CD11c (M1) and CD206 (M2) on macrophages was analyzed using a NC-3000. Data are shown as mean ± SEM. Statistical analysis was performed using one-way ANOVA and LSD post hoc tests. The comparisons between different culture mediums were done by t -tests. Statistically significant differences are indicated as ∗ p < 0.05, # p < 0.01, and † p < 0.001 versus vehicle control.

Article Snippet: Macrophages were incubated in the presence or absence of aspirin for 72 h and cultured in either control medium, the presence of LPS for the last 24 h of the incubation, or cocultured with 4T1 cells for 72 h. To assess surface marker expression, RAW 264.7 and 4T1 cells were collected after 72 h of coculturing and stained by incubating with fluorescein FITC anti-mouse CD11c and Alexa Fluor 647 anti-mouse CD206 monoclonal antibodies (Sony Biotechnology Inc.) at 4°C in the dark for 30 min. After washing, viable cells were stained with Hoechst 33342 (ChemoMetec, Allerød, Denmark) and subjected to FlexiCyte fluorescence-activated cell sorting analysis.

Techniques: Incubation, Cell Culture, Control